Forms, Policies & Procedures

Intravenous (IV) Tail Vein Injection SOP #PRO-005

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

SOP Concerning IV injection via the tail vein.

Live Rodent Transport SOP #PRO-017

Scope

This policy is applicable to all University of Delaware staff, researchers, students and technicians who provide care for and/or use animals for research.

Procedure

1. Rodents may be transported between animal housing and research laboratories on campus
2. Personnel transporting the rodents must be named on the approved protocol with the exception of vivarium staff.
3. Plans for transportation must be described in the approved protocol.
4. Primary Enclosure for Transport of Animals.
   • Limit exposures to allergens, waste products, and odors while providing sufficient air for animal respiration
   • Be the appropriate size for the animal being transported
   • be designed to prevent injury to the animals
   • contain the animals securely to prevent escape
   • contain animal waste so it is not released from the container
5. Mice may be placed in disposable containers provided by OLAM, or clean cages. Mice from different cages should be placed in separate containers to prevent fighting. Rats may be placed in clean cages. If animals are moved outside the vivarium, but stay within the building, the cages should be placed on a cart and covered. If animals are moved outside the building, containers or cages should be placed in a secure secondary container such as a tote bag or plastic bin with a lid and holes for ventilation.
6. Vehicles used for transportation must be approved by the Attending Veterinarian, and animals must be transported in a climate-controlled space. The animals must be transported directly to their destination.
7. Animals that are classified as biohazardous may require special precautions which must be described in the approved protocol.
8. Disposable containers may be discarded after use. Cages must be returned to the vivarium in a timely fashion.

Mouse Restraint – One Handed SOP #A-105

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

SOP Concerning the One Handed Restraint of mice.

Oral Gavage – Mouse & Rat SOP #A-106

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

Oral gavage is used to dose an animal with a specified volume of material directly into its stomach.

Retro-orbital Blood Collection – Mouse SOP #PRO-006

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

The mouse has a large retro orbital sinus than can be used as a source of venous blood by penetrating the sinus with a glass capillary tube.

Note: Anesthesia is required unless performing the procedure in an awake animal is justified and approved in the IACUC protocol.

Retro-Orbital Injection in Mice SOP #PRO-015

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

This is an alternative injection route to tail vein for intravenous injections. The injectable solution is limited to 200 µl or less per injection. Injections are not to be repeated in one day, unless you alternate eyes. Injections are limited to one injection per eye per day.

Training is required prior to performing this procedure.

Standard Operating Procedure Protocol Review

Institutional Animal Care and Use Committee (IACUC) Standard Operating Procedure: Protocol Review

Subcutaneous (SC or SQ) Injection SOP #PRO-007

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

The aim of this technique is to administer fluids or drugs into the space between the skin and the underlying tissue.

Submandibular Blood Collection – Mouse SOP #PRO-008

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

Mice have a small vascular bundle at the back of the jaw where the orbital vein, submandibular vein, and other facial veins join. This area can be punctured to obtain blood with minimal pain or distress.

Yield: up to 75µl

Survival Bleeding Volumes SOP #PRO-001

This Animal Subjects in Research procedure is in place to ensure the humane and ethical treatment of animal subjects in research. Please review the material carefully before proceeding.

SOP concerning the limitations of blood draws.